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Method for identifying olfactory receptor included in one olfactory cell

机译:一种嗅觉细胞中包含的嗅觉受体的鉴定方法

摘要

The present invention provides a novel method for identifying an olfactory receptor included in one olfactory cell. In the present invention, amplified is the cDNA derived from the mRNA of the one olfactory cell by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02. Subsequently, determined is whether or not a gene sequence of the amplified cDNA is identical to one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A. Finally, determined is that the olfactory receptor included in the one olfactory cell is the olfactory receptor corresponding to the one gene sequence which is identical to the gene sequence of the cDNA in the previous step, if the gene sequence of the cDNA is identical to the one gene sequence in the previous step.
机译:本发明提供了一种鉴定包含在一个嗅觉细胞中的嗅觉受体的新颖方法。在本发明中,使用SEQ ID:01代表的正向引物和SEQ ID:02代表的反向引物,通过PCR方法扩增了源自一个嗅觉细胞的mRNA的cDNA。随后,确定是否扩增的cDNA的基因序列与编码在小鼠嗅觉受体群A中的嗅觉受体的基因序列中包含的一个基因序列相同。最后,确定该嗅觉细胞中包含的嗅觉受体是与如果cDNA的基因序列与上一步中的一个基因序列相同,则该一个基因序列与上一步中的cDNA的基因序列相同。

著录项

  • 公开/公告号US8921048B2

    专利类型

  • 公开/公告日2014-12-30

    原文格式PDF

  • 申请/专利权人 PANASONIC CORPORATION;

    申请/专利号US201313784518

  • 发明设计人 MASATO SUZUKI;

    申请日2013-03-04

  • 分类号C12Q1/68;C12P19/34;

  • 国家 US

  • 入库时间 2022-08-21 15:17:16

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