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Method for immunizing animal, composition for immunization, method for producing antibody, method for producing hybridoma, and method for producing monoclonal antibody
Method for immunizing animal, composition for immunization, method for producing antibody, method for producing hybridoma, and method for producing monoclonal antibody
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机译:免疫动物的方法,免疫组合物,抗体的制备方法,杂交瘤的制备方法和单克隆抗体的制备方法
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摘要
It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.
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