Dual specific Protein Union directed against Interleukin 1B (il-1beta) and / or Interleukin 17 (IL - 17)

摘要

Multivalent binding proteins that bind to Multispecies manipulated and il-1b and / or il-17, together with Methods of Preparation and their use in the Prevention, diagnosis and / or treatment of a disease.Claim 1: a Binding Protein that comprises a First and a Second polypeptide Chain, each of which is independently vdu00b9 - (xu00b9) u2099 - vdu00b2 - C - X, whereVdu00b9 is a First variable domain is a domain variable vdu00b2; Second, C is a constant Domain; xu00b9 is a Connector with the condition not CH1; X is a FC region that is either present or absent; n is 0 OR 1,In Domains where vdu00b9 in said first and said Second polypeptide Chain forming a First Binding Site and the White vdu00b2 Functional Domains in the first and the second polypeptide Chain forming a Second White Functional binding site, and the binding protein. Ese the ability to join the il-1b and il-17, where(i) the variable domains that form a functional binding site for White il-1beta include: The COR 1 to 3 of SEQ ID no. 34 and the COR 1 to 3 of SEQ ID no. 35, the COR 1 to 3 of SEQ ID no. 32 and the COR 1 to 3 of the SEQ ID no. 33, the cor 1 to 3 of SEQ ID no. 36 and the COR 1 to 3 of the seq ID no. 37, the COR 1 to 3 of SEQ ID no. 38 and the COR 1 to 3 of SEQ ID no. 39The Cor or 1 to 3 of SEQ ID no. 40 and the COR 1 to 3 of SEQ ID no. 41; and / or binding protein has the ability to bind to the IL - 1B with a KD value of maximum 10u207bu00b9u00b2 approximately 5.1 x m, u1d50u1d49u1d48u2071u1d48u1d52 u1d56u1d52u02b3 u02b3u1d49u02e2u1d52u207fu1d43u207fu1d9cu2071u1d43 u1d48u1d49 u1d56u02e1u1d43u02e2u1d50u00f3u207f u02e2 u1d58u1d56u1d49u02b3u1da0u2071u1d9cu2071u1d43u02e1, u1d52 u02e1u1d43 u1d9cu1d43u1d56u1d43u1d9cu2071u1d48u1d43u1d48 u1d56u1d43u02b3u1d43 u2071u207fu02b0u2071u1d47u2071u02b3 u02e1u1d43 ilu207bu00b9b with a value of approximately 500 nm of maximum lcu2085u2080 as measured in a Neutralization Assay of IL - 1BAnd / or (ii) the variable domains that form a functional binding site for white include the il-17 COR 1 to 3 of SEQ ID no. 44 and the COR 1 to 3 of SEQ ID no. 45; The COR 1 to 3 of SEQ ID no. 42 and the COR 1 to 3 The SEQ ID no. 43; or The COR 1 to 3 of SEQ ID no. 46 and the COR 1 to 3 l To SEQ ID no. 47; and / or binding protein has the ability to bind to the IL - 17 with a KD value of COmo maximum approximately 4.8 m x 10u207bu00b9u00b2, u1d50u1d49u1d48u2071u1d48u1d52 u1d56u1d52u02b3 u02b3u1d49u02e2u1d52u207fu1d43u207fu1d9cu2071u1d43 u1d48u1d49 u1d56u02e1u1d43u02e2u1d50u00f3u207f u02e2u1d58u1d56u1d49u02b3u1da0u2071u1d9cu2071u1d43u02e1, u1d52 u1d57u2071u1d49u207fu1d49 u02e1u1d43 u1d9cu1d43u1d56u1d43u1d9cu2071u1d48u1d43u1d48 u1d56u1d43u02b3u1d43 u2071u207fu02b0u2071u1d47u2071u02b3 u02e1u1d43 ilu207bu00b9u2077 u1d9cu1d52u207f u1d58u207f u1d5bu1d43u02e1u1d52u02b3 u1d48 u1d49 icu2085u2080 maximum of approximately 1.7 nm, measured in a test of Neutralization of il-17.