Procedure for obtaining transgenic plants with high activity of GP, high content and yield in starch and high amylose / amylopectin ratio, expression vector, transformed or infected cell and use of the same, transgenic plant and use of the same, polyclonal antibodies or Monoc Lonais and use of the same

摘要

Process for the production of transgenic plants having high starch content and yield and a high amylose / amylopectin balance. The alpha-1,4-glucan phosphorylases (GPs) catalyze the reversible cutting of alpha-1,4 linkages of non-reducing ends of homopolysaccharides with at least 5 glucose molecules such as starch, maltodextrin and glycogen, leading to the production of glucose 1-phosphate. The GPs in bacteria and animal cells are responsible for the breakdown of glycogen. Although the increase in GP activity leads to a reduction in intracellular levels of glycogen in bacteria and animal cells in this invention to obtain plants having high levels and starch yields described, and a high amylose / amylopectin balance as result of the expression of genes coding for GPs.