Methods to determine in a large sample zigosidade

摘要

Methods to determine zigosidade in a sample are voluminous.The present invention relates to methods for determining the presence or absence of a sequence of nucleotides inserted in an insertion site of a nucleic acid specific and methods include: isolating a nucleic acid from a sample of bulky fabric; putting the acid n Ucleico in contact with an initiator directly able to bind to the nucleic acid upstream of the site of insertion.A first reverse primer specific for the sequence of nucleotides inserted, and a second reverse primer can bind to the nucleic acid downstream of the insertion site of the primers can be used to reproduce nucleic acids between the primers.The nucleic acids played can be analyzed to determine if a sequence of nucleotides inserted is present or absent in the sample.