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GENETICALLY ENGINEERED E. COLI EXPRESSING XYLANASE ENZYME

机译:基因工程化大肠杆菌表达木聚糖酶

摘要

This invention relates to an improved xylanase production by recombinant E. coli cells. Xylanase gene was isolated from Bacillus brevis, inserted into pET29a vector and expressed successfully in E. coli DH5a. The xylanase expression by transformed E. coli is found much better than the native Bacillus brevis strain and the xylanase enzyme is secreted directly into the medium. This improved production will reduce the cost of enzyme production at the commercial level.
机译:本发明涉及通过重组大肠杆菌细胞改进的木聚糖酶生产。从短小芽孢杆菌中分离出木聚糖酶基因,将其插入pET29a载体中并在大肠杆菌DH5a中成功表达。发现通过转化的大肠杆菌的木聚糖酶表达比天然短芽孢杆菌菌株好得多,并且木聚糖酶直接分泌到培养基中。这种改进的生产将在商业水平上降低酶生产的成本。

著录项

  • 公开/公告号IN2012DE01660A

    专利类型

  • 公开/公告日2014-12-05

    原文格式PDF

  • 申请/专利权人

    申请/专利号IN1660/DEL/2012

  • 发明设计人 AMRENDRA NATH PATHAK;GIRISH KUMAR GOSWAMI;

    申请日2012-05-31

  • 分类号

  • 国家 IN

  • 入库时间 2022-08-21 15:15:10

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