TWO STAGE ENZYME ASSAY FOR DETECTION OF LISTERIA MONOCYTOGENES IN MILK PRODUCTS

摘要

The present invention relates to the development of spore based assays for rapid detection of Listeria monocytogenesemploying combination of specific marker enzymes and germinogenic substrates. The invention also provides selective media for enrichment, enumeration and identification of target organism i.e. L. monocytogenespresents in a test sample.The assay is highly rapid and sensitive as it enables the detection of 10±2.0 cfu/mL ofL. monocytogenes within 3.30±0.30 h after pre-enrichment of test sample in LSEM for 16.0 ± 1.0 h.