The morphologically identified two distinct Argulus species, A. siamensis and A. japonicus were subjected to DNA extraction and amplification of 18s ribosomal gene. The obtained species specific sequences were obtained and two enzymes were found out based on their uniqueness in sequence information that will digest specifically both the species in unique manner producing bands of two different sizes. Based on the RFLP pattern, two species can be identified and differentiated that would help in easy and quicker diagnosis.
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