In the medical industry, the present invention provides a optical skin film sheet for biosensor fabrication to monitor human body tissues capable of producing an indicator response upon detection of the presence of certain functional chemical compounds in a biological and human body tissue samples. The present invention relates to devices and methods for detecting various analytes in a biological sample based upon the alteration of light characteristics associated with binding of the chemical composition. The optical characteristics of the fiber optic skin film transilluminator are experimented using LED light source and also LASER light of wavelength 650nm and 850nm. The voltage produced by the skin film sheet is 26mVolts to 356mVolts. When the light interacts with the skin film sheet at varies distance are recorded with the help of optical set up and the complete characteristics are described based on the present invention.
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