A molecular method to create a database for identification of high yielding plant varieties

摘要

Exemplary embodiments of the present disclosure are directed towards a molecular method to create a database for identification of a plant variety and its distinct quality traits such as yield, drought resistance, resistance to specific pests etc. Genomic DNA extracted from different varieties of banana plants were subjected to PCR amplification by using specific primers to get an amplicon. The resulting amplicon is then subjected to restriction digestion using different restriction endonucleases and resolved by gel electrophoresis. Different varieties of plants would exhibit different patterns of restricted fragments of DNA when resolved on a gel that are visually recorded to create a database. This database is used for easy identification of a particular variety of a plant.