The nanosized ZONps, spikes are developed through inexpensive and user friendly green chemistry routes. The process is carried out in the presence of aqueous acetate and aqueous hydroxide in the bi-distilled water followed with predetermined incubation periods. The solvent is stirred continuously during the process. The solution is cooled and has been undergone through predetermined incubation periods. Finally the desired nanosized ZONps, spikes solution is characterized and preserved safely. The synthesized ZONps spikes was further tested for the inhibition of Quorum sensing regulated virulence factors in different pathogens. Chromobclcterium Violaceum showed excellent reduced violacein production under the effect of the nanospikes as compared to untreated control. In the opportunistic pathogen P. aerugjnosa the synthesized nanospikes exhibited significantly impaired production of the QS regulated virulence factors. The ZONps spikes demonstrated outstanding reduction in virulence factors like Elastase, total protease, pyocyanin production and swarming motility. These results open a paradigm for ZnO nanoparticulates in future applications for the development of derivative agents to control the spread and infection of a variety of microbial strains.
展开▼
