METHOD FOR PREPARING POLYMERIC PROTEIN COMPOSED OF MONOMERIC PROTEIN PRODUCED BY FUSING PROTEIN HAVING IMMUNOGLOBULIN FOLD STRUCTURE TO PROTEIN CAPABLE OF SERVING AS SUBUNIT STRUCTURE

摘要

A method for preparing a polymeric protein which is composed of a monomeric protein produced by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure said method comprising: (A) a step of preparing the monomeric protein that has the form of granules insoluble in cells of a microorganism; (B) a step of solubilizing the monomeric protein prepared in step (A) in an aqueous solution containing lauroyl L Glu; (C) a step of diluting the solution prepared in step (B) with a buffer solution containing arginine hydrochloride to decrease the concentration of lauroyl L Glu; and (D) a step of replacing a solvent in the solution prepared in step (C) by a buffer solution by means of gel permeation chromatography or the like.