Probiotic formulation for dog oral health

摘要

Bacterial species belonging to Lactic acid bacteria are isolated from the healthy pups oral/aural/skin, in order to develop Probiotic formulation to be used for dog oral hygiene. The LAB were identified by 16s RNA sequencing and biochemical characters. All the isolates were tested for Probiotic characters like attachment to dog buccal epithelium, antimicrobial activity, antibiotic sensitivity, Hydrogen peroxide production, growth curves and ability to withstand spray drying. 5 isolates namely Lactobacillus plantarum MVC 22m, Enterococcus faecium MVC 15e , Pediococcus pentosaceus MVCl2m, Weisella cibariamvc 138s, Enterococcus munditii MVC le, were used in the formulation @ 1 billion organisms per dose for first 3 and 0.5 billion organisms per dose for the last 2 organisms. The stability period in the spry dried powder is 10 months. Liquid formulation in the form of spray has been developed. Clinical trials conducted in dogs to check the efficacy of this spray indicated that, it is effective against halitosis reduction, dental tartar reduction when treated for 4 days daily once or 4 sprays 5. day once. In vitro studies showed reduction in aerobic and anaerobic bacterial flora of the oral cavity and colonization of LAB. Keywords: ; - Lactic acid bacteria from dog oral cavity- Development of Probiotic formulation for dog"s oral hygiene- Clinical trials on efficacy of Probiotic formulation in dogs