UK - 2 biosynthetic gene and Method for improving productivity of UK 2 using the same

摘要

The Invention provides a production method with mass production capability of UK 2 at low cost, we analyzed Genomic DNA from 3 - 7 streptoverticillium sp., which produces UK - 2, to identify a region which is expected to be a group of biosynthetic genes of UK - 2. Moreover, by hybridizing Colonies were isolated successfully, the DNA in the region.In addition, we used the DNA to prepare a Strain in which altered genes in the region. It was found that the strain produces no UK - 2. It was verified that the genomic region outside the group of biosynthetic genes of UK - 2. In addition, became streptoverticillium sp. 3 - 7 by the introduction of a Vector in which to insert the biosynthetic genes of UK Group 2 isolates.It is also found that the productivity of UK - 2 by the Transforming Enhanced about 10 to 60 times or more compared with that of the parent Strain. Moreover, it was revealed that the two copies of the biosynthetic gene Cluster of UK - 2 were present in these transformants, respectively