RA3 plasmid replication system (mini repl RA3), sequences and a method for cloning and connecting the independent gene cassettes

摘要

the subject of the invention is a system replikacyjny ra3 plasmid (minireplikon ra3), sequence and cloning and laczenia niezaleznych gene cassettes.more more solution vectors plazmidowych concerns system based on stable minireplikon ra3 plasmid with a wide range of hosts with group of plasmids incu irregularities,znajdujacy applied in cloning, for storage, analysis the functional gene cassettes in different species of bacteria, and also their really easy skladaniu, laczeniu in any combinations - tasowaniu,purpose of the plasmids of pozadanych biotechnological characteristics.these vectors can be used for cloning and analysis of the function of genes, promoters bialek activity, overproduction in different host, also outside the family enterobacteriaceae.these vectors are the number of copies and koduja different selection markers in research in a broad range of hosts.