Method for preparation a thermosensitive coating substrate, the substrate with a thermosensitive coating and its application

摘要

The invention presented in this application relates to a substrate with a thermosensitive polymer layer of changeable affinity for water (LCST) within 0-80°C and the method by which it is made through the immobilization of a thermosensitive polymer from a group of homo- and copolymers of 2-substituted-2-oxazoline onto a modified surface consisting of a non-organic base substrate, preferably glass or silicon. The thermosensitive polymer is immobilized onto the substrate surface by the grafting of 2-substituted-2-oxazoline polymer chains and making the layer covalently bonded with the base substrate through the functional group of the silyl linker as indicated in formula 2 or 3. The polymer immobilization onto the base substrate is performed through grafting that initiates a cationic polymerization from the substrate or through termination of the polymerization by the functional groups on the substrate until a polymer brush conformation with a polymer grafting density of 0.1-1.8 chain/nm 2 is formed. The substrate with the thermosensitive layer made of a polymer from a group of homo- and copolymers of 2-substituted-2-oxazoline is used for cell culture, particularly skin cell culture.