Oligonucleotides, the nucleotide sequence of the gene fragment of the fusion protein F of Newcastle Disease virus (NDV), the use of the nucleotide sequence for the detection and differentiation of different strains of NDV

摘要

the subject invention are oligonucleotides, maintained nukleotydowa sequence from the gene encoding protein f, newcastle disease virus (discase newcastle virus, ndv, apmv - 1), the use of nucleotide sequences for detection and differentiation of strains ndv.oligonucleotides for dna sequence is characterized by the fact that serve to replicate protein gene fragments within ewolucyjnie strongly retained merger of this gene for viruses apmv - 1, belonging to the family paramyxoviridae, such avulavirus.dna sequence nukleotydowa characteristic that contains the sequence chosen from dna sequences or dna transkrybowanego with rna fragments of the gene coding for the fusion protein of newcastle disease virus contained in compartments 247 256 and 350 - 369 pairs calculated from the beginning of g newcastle disease virus as identified using enu f, ndv, apmv - 1.the invention also includes the use of dna nucleotide sequence primers, which is characterized by thewe used primers for duplication of gene encoding fusion protein of newcastle disease virus in the ewolucyjnie strongly retained fragment of this gene for detection and differentiation of different strains of newcastle disease virus and application ndv sequence n ukleotydowej replication of dna primers carried out rt-pcr reaction and examine the reaction productsseparated on agarose gel or polyacrylamide gel, and then stains dna and shall carry out a visual inspection in the radiation under a uv lamp.