Vector for expression of heterologous proteins in bacterial host cell, bacterial host cell and process for production of desired heterologous polypeptide

摘要

The invention relates to avector for the expression of heterologous proteins in a bacterial host cell, comprising at least one expression control sequence, and a nucleic acid molecule coding for a fusion protein, wherein the fusion protein comprises a first polypeptide which is the autoprotease Npro of a pestivirus, or a derivate thereof relating the Npro autoproteolytic activity, and includes a second polypeptide which is a heterologous polypeptide. The invention described a bacterial host cell and a process for the production of a desired heterologous polypeptide with clearly defined homogenous N-terminus in a bacterial host cells.