method for Modeling intrauterine infection of FETUS and NEWBORN as a consequence of sub-acute infectious-inflammatory MOTHER process

摘要

The invention relates to medicine, namely to pathological anatomy, obstetrics, gynecology, pediatrics, neonatology, microbiology, and relates to a method of modeling the intrauterine infection of the fetus and the newborn at the background of infectious and inflammatory mother process that includes three-time introduction of microorganisms Escherichia coli to female rats, and as a result of sub-acute infectious-inflammatory mother processes there is reproduced animal pre-sensitization with single subcutaneous introduction in the thigh of slurry of killed by heating for 1 hour at 80 ° C cells of reference strain Escherichia coli at a density of 1.0 units by McFarland scale, followed, after 10 days with performing the animal intra-abdominal infection with mixture of Escherichia coli bacterial culture in an amount of 1,5x109 in 20% mannitol solution under prior abdominal stimulation by introduction into the abdominal cavity of the animal of meat-peptone broth, infection is repeated after 10 and 20 days at similar conditions, then the female is fertilized, and the degree of affection of the internal organs of offspring is assessed by morphometric evaluation.