METHOD OF DETECTION OF DIROFILARIA IMITIS AND DIROFILARIA REPENS IN BIOLOGICAL SAMPLES BY POLYMERASE CHAIN REACTION

摘要

Method of detection of Dirofilaria imitis and Dirofilaria repens in biological samples by polymerase chain reaction consisting of PCR buffer preparation, amplification, detection amplifikatsiynoyi DNA. Use the genes 12S rRNA target containing such sequences of pairs of primers: 5 '-tttttgaccgggtttagtacc-3' 5 '-tgtgccaataaaattcaccaa-3' Product size 152 bp for Dirofilaria repens 5 '-tggcgcatgtatgattgttt-3' 5 '-tgacggatggtttgtaccac-3' Product size 472 bp for Dirofilaria imitis