SERUM INFECTION MARKERS FOR DIAGNOSIS OF TOXOPLASMOSIS

摘要

THE INVENTION RELATES TO A METHOD IN DIAGNOSING THE DISEASE TOXOPLASMOSIS IN HUMAN PATIENTS USING SERUM INFECTION MARKERS. THE INFECTION MARKERS WERE IDENTIFIED USING UNFRACTIONATED AND FRACTIONATED SERUM SAMPLES (DEPLETED OF 14 SERUM ABUNDANT PROTEINS) AND TECHNIQUES OF TWO-DIMENSIONAL GEL ELECTROPHORESIS, WESTERN BLOT, MASS SPECTROMETRY AND AFFINITY CHROMATOGRAPHY. AMONG PROTEINS WHICH HAVE BEEN IDENTIFIED WERE TWO HOST SPECIFIC PROTEINS AND FIVE PARASITE-SPECIFIC PROTEINS. THESE SERUM INFECTION MARKERS FROM SAMPLES OF PATIENTS WITH EVIDENCE OF ACTIVE TOXOPLASMOSIS CAN SERVE AS ANTIGEN DETECTION TEST(S) FOR TOXOPLASMOSIS DIAGNOSIS. FIGURE 1 (B)