Method for obtaining osteoblasts allograft enriched with a patient

摘要

Method for obtaining an allograft enriched osteoblasts of a patient comprising (a) culturing pieces of bone of said patient in the mid-high glucose DMEM (4500 mg / L glucose), 1% glutamine, 1% pyruvate, penicillin / streptomycin 1% and 20% blood serum of said patient for 3-4 weeks to obtain a culture of osteoblasts, (b) adding trypsin and defined medium in step (a) and centrifuging, removing the supernatant, (c) seeding osteoblasts obtained in step (b) and repeated at 5-9 days of step (b), (d) culturing the osteoblasts obtained in step (c) fragments of human bone allograft in the presence of defined medium in step (a) incubating for 40-56 hours and centrifuging, removing the supernatant, adding serum obtained from patient, centrifuging and discarding the supernatant, to obtain the osteoblast enriched allograft patient.