Two-stage temperature profile for virus spread

摘要

Method for the production of a virus, the method comprising: providing a host cell infected by the virus, culturing the infected host cell at a first temperature between 31 ° C and 37 ° C for 1 to 48 hours, then culturing the infected host cell at a second temperature that is 1ºC to 6ºC lower than the first temperature, and collect copies of the virus produced by the culture steps, said virus consisting of an orthomyxovirus, alphavirus or flavivirus, the method being carried out on an industrial scale in more than 50 liters of cell culture and the method having a positive effect on the purity of the antigen compared to the same method carried out at a constant temperature.