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Use of human myeloid leukemic cells for antibody expression

机译:人类骨髓白血病细胞用于抗体表达的用途

摘要

A method of producing a molecular protein composition, comprising (a) introducing into a host cell that is an immortalized human blood cell at least one nucleic acid encoding at least a portion of said protein; and (b) culturing said host cell under conditions that allow the production of said protein molecular composition; and (c) isolate said protein molecular composition. wherein the host cell is selected from the group consisting of DSM ACC2858 (GT-2X), DSM ACC 2806 (NMH9D8), DSM ACC 2807 (NM-H9D8-E6), DSM ACC 2856 (NM-H9D8-E6Q12) or a cell or cell line derived from them.
机译:一种生产分子蛋白质组合物的方法,包括:(a)将至少一种编码至少一部分所述蛋白质的核酸引入到永生化的人血细胞的宿主细胞中; (b)在允许产生所述蛋白质分子组成的条件下培养所述宿主细胞; (c)分离所述蛋白质分子组成。其中宿主细胞选自DSM ACC2858(GT-2X),DSM ACC 2806(NMH9D8),DSM ACC 2807(NM-H9D8-E6),DSM ACC 2856(NM-H9D8-E6Q12)或细胞或衍生自它们的细胞系。

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