Microorganisms busiest sucrose mutase

摘要

microbial cell with a synthesis rate isomaltulose at least 2 times higher than the wild type, which presents in the genome a smuA gene encoding a mutase sucrose SmuA so that at least one promoter smuA element is replaced by the least 5 other promoter (promoter ersatz) chosen from: a) an endogenous gene promoter 3,4-dihydroxy-2-butanon-4-phosphate synthase (ribB) b) a homologous promoter of the endogenous promoter as a) from a foreign donor strain and c) a functional fragment of the promoter a) or b), wherein the promoter substitute is characterized by a selected polynucleotide of the group consisting of polynucleotide molecules with a nucleotide sequence according to SEQ ID NO: 2 to SEQ ID NO: 3 and homologous sequences thereof with at least 85% identity.