SURFACE IMMOBILIZED POLYELECTROLYTE WITH MULTIPLE FUNCTIONAL GROUPS CAPABLE OF COVALENTLY BONDING TO BIOMOLECULES

摘要

A polyelectrolyte having multiple exposed functional groups, each such groupbeing capable of covalently bonding to a molecule, is immobilized on a surfacefor the purpose of bonding to a biomolecule. The biomolecule can be, forexample, a nucleic acid, e.g., an amine functionalized oligonucleotide. Thepolyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is boundto a functionalized surface using a covalent immobilization strategy, e.g.,reaction with the surface of a tosyl-activated microparticle. Following suchreaction, exposed reactive functional groups on the protein, such as amine,carboxyl, thiol, hydroxyl groups can further be utilized to covalently couplethe oligonucleotide of interest using suitable chemistry.