Gram negative bacterial virulence genes are identified, thereby allowing the identification of novel anti-bacterial agents that target these virulence genes and their products, and the provision of novel gram negative bacterial mutants useful in vaccines. The invention comprises an isolated, attenuated Pasteurellaceae bacterium selected from the group consisting of Pasteurella (Mannheimia)haemolytica, Pasteurella multocida, and Actinobacillus pleuropneumoniae comprising a mutation in a polynucleotide sequence that encodes a yleA polypeptide comprising an amino acid sequence at least 70% identical to the yleA amino acid sequence of SEQ ID NO: 81, wherein the mutation results in decreased virulence and attenuation of the bacterium.
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机译：鉴定出革兰氏阴性细菌毒力基因，从而允许鉴定靶向这些毒力基因及其产物的新型抗菌剂，并提供可用于疫苗的新型革兰氏阴性细菌突变体。本发明包括一种分离的减毒的巴斯德杆菌属细菌，其选自巴斯德氏菌（Mannheimia）溶血性巴氏杆菌，多杀性巴斯德氏菌和胸膜肺炎放线杆菌，其在编码yleA多肽的多核苷酸序列中包含突变，所述yleA多肽包含与氨基酸至少有70％相同的氨基酸序列。 SEQ ID NO：81的yleA氨基酸序列，其中所述突变导致细菌的毒力降低和减毒。
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