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THERMOSTABLE PEROXIDE-DRIVEN CYTOCHROME P450 OXYGENASE VARIANTS AND METHODS OF USE
THERMOSTABLE PEROXIDE-DRIVEN CYTOCHROME P450 OXYGENASE VARIANTS AND METHODS OF USE
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机译:热稳定的过氧化物驱动的细胞色素P450氧化酶变体及其使用方法
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摘要
The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.
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