首页> 外国专利> MORTIERELLA ALPINE URACIL AUXOTROPH WITH URA5 GENE KNOCKED OUT THROUGH HOMOLOGOUS RECOMBINATION, AND CONSTRUCTION METHOD THEREOF

MORTIERELLA ALPINE URACIL AUXOTROPH WITH URA5 GENE KNOCKED OUT THROUGH HOMOLOGOUS RECOMBINATION, AND CONSTRUCTION METHOD THEREOF

机译：通过同源重组技术剔除出具有URA5基因的莫特尔菌素尿嘧啶营养缺陷型及其构建方法

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摘要

The present invention relates to a Mortierella alpine ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention, Mortierella alpine ATCC32222 is used as a material and undergoes gene knockout through an Agrobacterium tumefaciens mediated genetic manipulation technology, to obtain the Mortierella alpine uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus Mortierella alpine ATCC32222 and product development.

机译：本发明涉及莫氏菌高山ATCC32222尿嘧啶营养缺陷型菌株及其构建方法。在本发明中，以高山被孢霉为材料，通过根癌农杆菌介导的基因操作技术进行基因敲除，得到高山被孢霉的营养缺陷型。该方法对产油真菌高山铁线虫ATCC32222的基础理论研究和产品开发具有重要意义。

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公开/公告号WO2015018198A1

专利类型
公开/公告日2015-02-12

原文格式PDF
申请/专利权人 JIANGNAN UNIVERSITY;
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申请/专利号WO2014CN72350
发明设计人 ZHANG HAO;DU KAI;CHEN YONGQUAN;ZHAO JIANXIN;HUANG XIAOYUN;GU ZHENNAN;CHEN WEI;ZHAO SHANSHAN;HAO GUANGFEI;CHEN HAIQIN;
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申请日2014-02-21
分类号C12N1/15;C12N15/09;
国家 WO
入库时间 2022-08-21 15:08:25

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