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Methods for detecting nucleic acid with microarray and program product for use in microarray data analysis

机译:用微阵列检测核酸的方法和用于微阵列数据分析的程序产品

摘要

PROBLEM TO BE SOLVED: To provide a microarray-based method capable of detecting, with high accuracy from an analyte sample, a target nucleic acid containing a base sequence to which a nucleic acid-binding protein binds.SOLUTION: In the method, a signal is obtained from a probe not containing any sequence complementary to the base sequence to which the nucleic acid-binding protein binds for being used as a background to correct a signal obtained from a detection probe hybridizing to the target nucleic acid.
机译:解决的问题:提供一种基于微阵列的方法,该方法能够从分析物样品中高精度检测包含核酸结合蛋白结合的碱基序列的靶核酸。解决方案:在该方法中,信号从不包含与核酸结合蛋白所结合的碱基序列互补的任何序列的探针中获得的DNA作为背景,以校正从与目标核酸杂交的检测探针获得的信号。

著录项

  • 公开/公告号EP2270747B1

    专利类型

  • 公开/公告日2015-05-06

    原文格式PDF

  • 申请/专利权人 SYSMEX CORP;

    申请/专利号EP20100167894

  • 申请日2010-06-30

  • 分类号G06T7/00;G01N33/48;C12Q1/68;

  • 国家 EP

  • 入库时间 2022-08-21 15:08:19

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