METHOD FOR PROMOTING FORMATION OF HEPATITIS B SURFACE ANTIGEN COVALENTLY CROSSLINKED PARTICLES EXPRESSED BY SACCHAROMYCES CEREVISIAE

摘要

Provided in the present invention is a method for promoting the formation of hepatitis B surface antigen covalently crosslinked particles expressed by Saccharomyces cerevisiae, comprising the following steps: maintaining recombinant hepatitis B surface antigens expressed by Saccharomyces cerevisiae under the condition of a temperature of 4-100°C for 3 months to 5 minutes, to make polypeptides, which are non-covalently bound to each other, inter-chains and dimers formed by single polypeptides convert to disulfide bonds between polypeptides, and forming covalently crosslinked antigen particles. The present invention can promote the formation of high immunity antigen particles without the addition of chemicals.