ENGINEERING DNA ASSEMBLY IN VIVO AND METHODS OF MAKING AND USING THE REVERSE TRANSCRIPTASE TECHNOLOGY

摘要

Cells that can synthesize oligonucleotides in vivo to produce a nucleic acid nanostmcture are described. Methods for producing oligonucleotide nano structures for use in regulating gene expression and altering biological pathways are provided. Methods of performing multiplex automated genome editing (MAGE) are also provided. Methods of synthesizing single stranded DNA (ssDNA) comprising expressing a reverse transcriptase in a cell and a functional template comprising a non-coding tRNA structure at the 3' end and a coding RNA sequence at the 5' end, wherein the non-coding tRNA structure is capable of initiating transcription of the coding RNA sequence using the reverse transcriptase to produce the ssDNA oligonucleotide in the cell are also provided.