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SELF-REPRODUCING HYBRID PLANTS

机译:自我繁殖的混合植物

摘要

Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe clonal diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe clonal diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the male diploid genome in the zygote and normal development of the endosperm. Additionally provided are plants and seeds produced by the methods of the invention.
机译:提供了用于产生自繁殖杂种植物的组合物和方法。组合物包括编码多核苷酸和启动子的抑制盒,其产生MiMe克隆二倍体配子表型组合物,抑制盒和表达盒可用于基因组消除受精合子中的亲本二倍体配子。该方法包括将第一植物与第二植物杂交,该第一植物包括负责产生MiMe克隆二倍体配子表型的第一抑制盒和表达活性CENH3突变体的第一表达盒,该第二植物包括降低野生型CENH3水平的第二抑制盒。第二表达盒包含在胚珠中特异性表达CENH3的多核苷酸。所得子代植物的自体受精导致合子中雄性二倍体基因组的消除和胚乳的正常发育。另外提供了通过本发明的方法产生的植物和种子。

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