CULTURE MEDIA FOR IN VITRO DIFFERENTIATION OF CARDIOMIOCYTES FROM ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS AND METHOD TO SELECT THEM

摘要

It is disclosed a method to assess cardiogenic differentiation in adipose tissue-derived stromal cells (ASCs) and to select growth media with enhanced capability to stimulate cardiocyte differentiation of ASCs, based on assessing the enhanced expression of a selected pool of markers consisting of NKX2-5, MEF2C, HAND2, HAND1, CAMTA and FOG genes/transcription factors. Growth media selected by this method, containing a mixture of suitable growth factors and cytokines are also disclosed, showing a high cardiogenic differentiation potency of ASCs.