DEEP-CELL SUPER-RESOLUTION IMAGING METHOD AND SYSTEM AND PRISM OPTICAL SHEET APPARATUS

摘要

A deep-cell super-resolution imaging method and system and a prism optical sheet apparatus are provided. The imaging method uses super-resolution optical fluctuation imaging (SOFI) and super-resolution localisation microscopy (LM), can acquire deep-cell super-resolution imaging by using a computer to eliminate background noise, and can be directly applied to an ordinary fluorescent microscope without requiring modification to the original optical structure. The imaging system loads the prism optical sheet apparatus onto an inverted microscope and uses a physical method to reduce the background noise, obtaining deep-cell super-resolution images by means of localisation microscopy, and can be directly loaded on to a traditional inverted fluorescent microscope.