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ADIPOSE-DERIVED STEM CELL SHEET HAVING BONE DIFFERENTIATION POTENTIAL, AND METHOD FOR PRODUCING SAME

机译:具有骨分化潜能的脂肪来源的干细胞片及其制备方法

摘要

[Problem] Provided are: an easy, inexpensive method for producing a cell construct that can serve as a bone tissue restoration material; and a cell construct having osteogenic potential obtained by this method. [Solution] A method for producing a single-layer adipose-derived stem cell sheet having bone differentiation potential, the method comprising adding a solution, prepared by adding ascorbic acid or a salt thereof to cell culture medium free of cell differentiation inducer, to a culture container having no scaffold added thereto and culturing adipose-derived stem cells in this solution; and an adipose-derived stem cell sheet produced by this method.
机译:[问题]提供:一种简单,便宜的方法,用于生产可用作骨组织修复材料的细胞构建体;通过这种方法获得的具有成骨潜力的细胞构建体。 [解决方案]制备具有骨分化潜能的单层脂肪干细胞片的方法,该方法包括向无细胞分化诱导剂的细胞培养基中添加通过添加抗坏血酸或其盐而制备的溶液。不添加支架的培养容器,在该溶液中培养脂肪来源的干细胞。以及通过该方法制造的脂肪来源的干细胞片。

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