首页>
外国专利>
METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED CELL DERIVED DNA AS A MEASURE OF CELL LOSS IN DIABETES
METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED CELL DERIVED DNA AS A MEASURE OF CELL LOSS IN DIABETES
展开▼
机译:基于探针的PCR检测方法测量循环的脱甲基化细胞衍生DNA水平作为衡量糖尿病患者细胞损失的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for measuring blood levels of β cell DNA that is released upon β cell death by using a quantitative probe technology to detect amplified methylated and demethylated forms of the insulin gene DNA, representing normal tissue and β cell specific origin, respectively. Using probes permits the sensitive and specific identification of demethylated insulin DNA patterns that are present only in β cells. The method offers a bioassay for detecting β cell loss in diabetes, useful for screening of prediabetes, monitoring of disease progression, and selection and monitoring of therapies. The technique finds potential use in both Type I and Type II diabetes, as well as gestational diabetes.
展开▼