A NUCLEIC ACID DETECTION METHOD COMPRISING TARGET SPECIFIC INDEXING PROBES (TSIP) COMPRISING A RELEASABLE SEGMENT TO BE DETECTED VIA FLUORESCENCE WHEN BOUND TO A CAPTURE PROBE

摘要

Reagents, systems and methods for the detection of nucleic acids are described herein, including such methods which may be performed in a single reaction. Such reagents include a target specific indexing probe (TSIP) synthetic DNA structure comprising a detectable moiety and a quencher thereof, wherein in the presence of a target nucleic acid a portion (a tag) of the (TSIP) synthetic DNA structure comprising the detectable moiety is released, thereby increasing the signal emitted therefrom. The tag may in turn bind to a capture probe, facilitating signal detection.