A microfluidic impedance flow cytometer ('MIC') device (2) comprises a substrate (4) in which is formed at least one flow channel (6) for leading through a particle (22) containing fluidic sample. The flow channel (6) is formed with a focusing zone (12) and a measurement zone (14) located downstream of the focusing zone (12) in the direction of through flow and provided with an electrode arrangement (18) for characterising particles (22) in the flowing fluidic sample by means of electrical impedance wherein an acoustophoretic particle focusing arrangement (20) is provided in acoustic coupling to the flow channel (6) in the focusing zone (12) to induce acoustic forces in fluid in the flow channel (6) so as to effect a lateral and/or vertical focusing of particles before flowing to the measurement zone (14).
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