The present invention relates to a bio probe for detecting swine fever virus, comprising (a) a nanomaterial; (b) an antibody which is specifically combined with an antigen of swine fever virus covalently linked to the surface of the nanomaterial; and (c) a glass fiber, as a solid substrate, having a surface which is modified by the antibody combined nanomaterial. The present invention also relates to a method for diagnosing swine fever virus using the bio probe. According to the present invention, the existence and infection of swine fever virus can be rapidly checked within 10 minutes using a small amount of a sample.
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