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A freezing medium composition for cryopreserving amniotic fluid-derived stem cells and a method for cryopreserving the same
A freezing medium composition for cryopreserving amniotic fluid-derived stem cells and a method for cryopreserving the same
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机译:用于冷冻保存羊水的干细胞的冷冻培养基组合物及其冷冻方法
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摘要
The present invention can greatly reduce the Me 2 SO concentration and remove animal serum (fetal bovine serum) while using amylase, sucrose and catalase to cryopreserve long-term cryopreservation With the method, amniotic fluid stem cells can be cryopreserved using standard 1/4 Me 2 SO concentration by adding disaccharides, antioxidants and a caspase inhibitor. As a result, the use of low concentration Me 2 SO 4 in cryopreservation solutions has the industrial effect of supporting clinical development of AFSC.
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机译:利用淀粉酶,蔗糖和过氧化氢酶冷冻保存长期冷冻保存,本发明可以大大降低Me 2 Sub> SO的浓度,去除动物血清(胎牛血清)。通过添加二糖,抗氧化剂和半胱天冬酶抑制剂,以标准的1/4 Me 2 Sub> SO浓度冷冻保存。因此,在冷冻保存溶液中使用低浓度的Me 2 Sub> SO 4 Sub>具有工业效应,可支持AFSC的临床发展。
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