METHOD FOR THE PRODUCTION OF SECOL DERIVATIVES BY ENANTIOSELECTIVE ENZYMATIC REDUCTION OF SECODIONE DERIVATIVES BY MEANS OF OXIDOREDUCTASE/DEHYDROGENASE IN THE PRESENCE OF NADH OR NADPH

摘要

The present invention relates to an enantiomerically selective enzymatic reduction method of secodione derivatives of general formula I wherein the ecdecione derivatives are reduced using an oxidoreductase / dehydrogenase enzyme in the presence of NADH or NADPH as cofactors. In the present invention, the ecdecoin derivative is used in a concentration of = 10 g / L in the reaction batch and the oxidized cofactor NAD or NADP formed by the oxidoreductase / dehydrogenase is continuously regenerated: In the above Formula I, The ring structure may not contain heteroatoms, or may include one or several, R ₁ is hydrogen or a C ₁ -C ₄ alkyl group, R ₂ is a conventionally known protecting group for OH, such as hydrogen, a C ₁ -C ₈ alkyl group or an ester, R ₃ is hydrogen, a methyl group or a halide, Structural element Is a benzene ring or a C ₆ ring having 0, 1 or 2 CC double bonds, The double bond is optionally present at the 6/7 or 7/8 position, and The carbons in positions 1, 2, 4, 5, 6, 7, 8, 9, 11, 12 and 16 are independently substituted with hydrogen, a C ₁ -C ₄ alkyl group, a halide or a phenyl group.