IMMOBILIZATION METHOD OF LIPASE ORIGINATE FROM RHIZOMUCOR MIEHEI USING NANO SILICA CARRIER CONTAIN HYDROPHOBIC BUTYL FUNCTIONAL GROUP

摘要

The present invention relates to a method for preparing a silica carrier using an immobilization technique in order to immobilize lipase to a carrier with a 30-40 nm of nanopore structure using a biocatalyst and to repetitively use the same for manufacturing low trans fats and processing fats in an environment-friendly method. Accordingly, the present invention: bonds 1-10% of hydrophobic butyl silane to 30-40 nm of silica carrier under pH 1.0-10.0; protects an active site of the lipase by adding 2.0-5.0% of soybean oil to lipase originated from Rhizomucor miehei; and immobilizes under a reaction condition of pH 3.0-9.0. The present invention coats the immobilized lipase with 0.2-2.0% of polyethylene imine, thereby protecting the active site of the lipase and enhancing stability. By improving the conventional chemical methods to an environment-friendly method using the biocatalyst: wastewater generated when processing fats can be reduced; processes can be simplified; and stability of products can be improved. Also, the present invention facilitates repetitive use by immobilization of lipase, thereby reducing manufacturing costs.