METHODS FOR MEASURING POLYMERASE ACTIVITY USEFUL FOR SENSITIVE, QUANTITATIVE MEASUREMENTS OF ANY POLYMERASE EXTENSION ACTIVITY AND FOR DETERMINING THE PRESENCE OF VIABLE CELLS

摘要

When returning a purified or selected cell type may be used to count a prokaryotic cell, through combination with the bead cytolytic new has the capability to measure reproducibly a DNA polymerase extension activity from less than 10cfu of bacteria this is a very sensitive and rapid quantitative DPE-PCR analysis is disclosed. In addition, Gram-negative bacteria, Gram-DPE-PCR analysis of the potential of the invention by testing a panel of microorganisms consisting of bacteria, positive and Candida species are universally detecting a microorganism is disclosed. Furthermore, DPE-PCR analysis of the present invention can be used to assess the bacterial cell viability, it is disclosed that they are provided through a strong correlation reproducible between proliferation represented by the presence of the DNA polymerase extending the active and cfu. Disclosed the analysis of the present invention believe that a useful quantitative tools for test purposes in a wide range of pharmaceutical, environmental, food and clinical environment. ;