Manufacturing method of virus-like particle vaccine against canine influenza virus

摘要

The present invention relates to a manufacturing method of a vaccine against canine influenza virus capable of preventing and/or treating canine influenza virus and, more specifically, to a manufacturing method of a VLP (virus-like particle) vaccine against canine influenza virus, to the VLP vaccine against canine influenza virus manufactured by the method, and to a method for preventing canine influenza virus by inoculating dogs with the VLP vaccine against canine influenza virus manufactured by the manufacturing method. The manufacturing method of the VLP vaccine against canine influenza virus comprises: a step for amplifying hemagglutinin (HA) genes of the canine influenza virus with a sequence number 1 by a forward primer with a sequence number 2 and a reverse primer with a sequence number 3, and for PCR-amplifying matrix 1 (M1) genes of the canine influenza virus with a sequence number 4 by a forward primer with a sequence number 5 and a reverse primer with a sequence number 6; a step for cloning the amplified HA genes and M1 genes to a vector; and a step for VLP-manifesting the vector cloned with the amplified HA genes and M1 genes in insect cells.