首页>
外国专利>
METHODS OF REDUCING LEVEL OF ONE OF MORE IMPURITIES IN A SAMPLE DURING PROTEIN PURIFICATION
METHODS OF REDUCING LEVEL OF ONE OF MORE IMPURITIES IN A SAMPLE DURING PROTEIN PURIFICATION
展开▼
机译:减少蛋白质纯化过程中样品中一种或多种杂质的降低水平的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A flow-through process for purifying a target molecule from a Protein A eluate comprising the steps of:(i) contacting the eluate recovered from a Protein A chromatography column with activated carbon, (ii) contacting the flow-through sample from step (i) with an anion exchange chromatography media, (iii) contacting the flow-through sample from step (ii) with a cation exchange chromatography media and (iv) obtaining the flow-through sample from step (iii) comprising the target molecule, wherein the eluate flows continuously through steps (i)-(iii) and wherein level of one or more impurities in the flow-through sample after step (iii) is lower than the level in the eluate in step (i). The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.
展开▼