METHOD OF DETECTING A MARKER FOR DIAGNOSING A DESEASE USING A COMPETETIVE IMMUNOASSAY BASED ON A SURFACE-ENHANCED RAMAN SCATTERING

摘要

The present invention is to prepare the sample solution; Preparing a specific antibody is immobilized by the magnetic particles to bind to the antigen that corresponds to the disease marker, and; Preparing a hollow gold nanoparticles bound antigen and Raman reporter molecules and the marker for the disease; It was added to the antibody-immobilized magnetic particles in the sample liquid, leading to a first immune reaction between the sample solution and the antibody immobilized to the magnetic particles; After the first immune response is induced, by the addition of gold nanoparticles in the hollow the sample solution, the sample solution is guided to a second immune response to an immune response of the magnetic particles and the gold nanoparticles and the hollow competitive and ; And the second immune reaction is complete, the filtrate of the sample is irradiated with a laser beam, except for the magnetic particles; And the laser light irradiation since the surface-enhanced Raman scattering (surface-enhanced Raman scattering: SERS) measure the signal confirming the marker; service-based SERS detection method using the diagnostic marker of the disease, the immune response, including competition The. ;