An organic solvent-tolerant endo-1,4-amp;bgr;-mannanase gene EM17 and recombination mannannase from transformed strain using thereof

摘要

The invention dongae or the like to decompose the garbage effectively: The transformed with a recombinant vector, the recombinant vector containing (Blacksoldier fly BSF) lyase artificial Chapter culture that can not be met novel microorganism derived from larvae gene EM17, the gene trait relates to a switching element. Lyase met EM17 of the present invention are expressed in E. coli in high concentration of organic solvents, high metal ion and a wide range of acid or base concentration exert resolution it is possible to reduce the unnecessary purification step in the recycling process of biomass hemicellulose. In addition, since various kinds of hydrolytic functional foods, animal feed, brewing, paper. Pulp industry, and it can be used for oligosaccharide synthesis. ;