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Methods for anylysising target protein using PYP proteins and PYP mutants therefor

机译:利用PYP蛋白及其PYP突变体解析靶蛋白的方法

摘要

The present invention relates to a protein expression or quantitative analysis method using PYP protein and PYP protein for protein expression or quantitative analysis. The protein expression or quantitative analysis method according to the present invention can quickly and accurately recognize the expression of a target protein, The purity can be measured. Further, the present invention does not cause any problems even when a tag system is used in combination, and can be applied not only to prokaryotes but also to eukaryotic protein expression systems. Because of this property, it has the utmost usefulness for ultra-rapid protein expression screening. Therefore, the protein expression or quantitative analysis method of the present invention can be usefully utilized in related studies of proteins.
机译:本发明涉及使用PYP蛋白和PYP蛋白进行蛋白表达或定量分析的蛋白表达或定量分析方法。根据本发明的蛋白质表达或定量分析方法可以快速且准确地识别目标蛋白质的表达,可以测量纯度。此外,即使将标记系统组合使用,本发明也不会引起任何问题,并且不仅可以应用于原核生物,而且可以应用于真核蛋白质表达系统。由于此特性,它对超快速蛋白质表达筛选具有最大的实用性。因此,本发明的蛋白质表达或定量分析方法可以有效地用于蛋白质的相关研究中。

著录项

  • 公开/公告号KR101527766B1

    专利类型

  • 公开/公告日2015-06-11

    原文格式PDF

  • 申请/专利权人 한국과학기술원;

    申请/专利号KR20130021003

  • 发明设计人 이효철;김영민;

    申请日2013-02-27

  • 分类号C07K19;C12N15/12;C12N15/63;G01N33/68;

  • 国家 KR

  • 入库时间 2022-08-21 14:58:03

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