METHOD AND KIT FOR IDENTIFYING LARIMICHTHYS POLYACTIS AND LARIMICHTHYS CROCEA USING REAL-TIME PCR

摘要

The present invention provides a technique to specifically, rapidly, and accurately discriminate Larimichthys polyactis and Larimichthys crocea by performing real-time PCR using a real-time PCR primer pair and a probe respectively specific to Larimichthys polyactis and Larimichthys crocea. In the present invention, Larimichthys polyactis and Larimichthys crocea are simply, rapidly, accurately, and specifically discriminated by using real-time PCR, so that a countermeasure for rapidly blocking that Chinese Larimichthys crocea is disguised as Larimichthys polyactis is ensured during import, quarantine, and distribution processes. Accordingly, transparency is promoted in a distribution process of Larimichthys polyactis and Larimichthys crocea, and benefits and health of domestic consumers may be protected.